Development and standardization of a rapid, PCR-based method for the detection of Wuchereria bancrofti in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis.
Identifieur interne : 009476 ( Main/Exploration ); précédent : 009475; suivant : 009477Development and standardization of a rapid, PCR-based method for the detection of Wuchereria bancrofti in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis.
Auteurs : S A Williams [États-Unis] ; S J Laney ; L A Bierwert ; L J Saunders ; D A Boakye ; P. Fischer ; D. Goodman ; H. Helmy ; S L Hoti ; V. Vasuki ; P J Lammie ; C. Plichart ; R M R. Ramzy ; E A OttesenSource :
- Annals of tropical medicine and parasitology [ 0003-4983 ] ; 2002.
Descripteurs français
- KwdFr :
- ADN des helminthes (analyse), Animaux, Culicidae (parasitologie), Filariose lymphatique (épidémiologie), Humains, Lutte contre les moustiques (), Prévalence, Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (normes), Vecteurs de maladie, Wuchereria bancrofti (isolement et purification).
- MESH :
- analyse : ADN des helminthes.
- isolement et purification : Wuchereria bancrofti.
- normes : Réaction de polymérisation en chaîne.
- parasitologie : Culicidae.
- épidémiologie : Filariose lymphatique.
- Animaux, Humains, Lutte contre les moustiques, Prévalence, Réaction de polymérisation en chaîne, Vecteurs de maladie.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Helminth.
- epidemiology : Elephantiasis, Filarial.
- isolation & purification : Wuchereria bancrofti.
- methods : Mosquito Control, Polymerase Chain Reaction.
- parasitology : Culicidae.
- standards : Polymerase Chain Reaction.
- Animals, Disease Vectors, Humans, Prevalence.
Abstract
PCR has recently been studied as a promising tool for monitoring the progress of efforts to eliminate lymphatic filariasis. PCR can be used to test concurrently at least 30 pools, with as many as 40 mosquitoes in each pool, for the presence of filarial larvae. The SspI PCR assay for the detection of Wuchereria bancrofti DNA in pools of mosquitoes has been used since 1994 in a variety of laboratories worldwide. During that time, the original assay has been modified in these different laboratories and no standardized assay currently exists. In an effort to standardize and improve the assay, a meeting was held on 15-16 November 2001, at Emory University in Atlanta, with representatives from most of the laboratories currently using the assay. The first round of testing was designed to test the four most promising methods for DNA extraction from pools of mosquitoes. Two of the four methods stood out as clearly the best and these will be now optimised and evaluated in two further rounds of testing.
DOI: 10.1179/000349802125002356
PubMed: 12625916
Affiliations:
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Fischer</name></author><author><name sortKey="Goodman, D" sort="Goodman, D" uniqKey="Goodman D" first="D" last="Goodman">D. Goodman</name></author><author><name sortKey="Helmy, H" sort="Helmy, H" uniqKey="Helmy H" first="H" last="Helmy">H. Helmy</name></author><author><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name></author><author><name sortKey="Vasuki, V" sort="Vasuki, V" uniqKey="Vasuki V" first="V" last="Vasuki">V. Vasuki</name></author><author><name sortKey="Lammie, P J" sort="Lammie, P J" uniqKey="Lammie P" first="P J" last="Lammie">P J Lammie</name></author><author><name sortKey="Plichart, C" sort="Plichart, C" uniqKey="Plichart C" first="C" last="Plichart">C. Plichart</name></author><author><name sortKey="Ramzy, R M R" sort="Ramzy, R M R" uniqKey="Ramzy R" first="R M R" last="Ramzy">R M R. Ramzy</name></author><author><name sortKey="Ottesen, E A" sort="Ottesen, E A" uniqKey="Ottesen E" first="E A" last="Ottesen">E A Ottesen</name></author></analytic><series><title level="j">Annals of tropical medicine and parasitology</title><idno type="ISSN">0003-4983</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Culicidae (parasitology)</term><term>DNA, Helminth (analysis)</term><term>Disease Vectors</term><term>Elephantiasis, Filarial (epidemiology)</term><term>Humans</term><term>Mosquito Control (methods)</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase Chain Reaction (standards)</term><term>Prevalence</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (analyse)</term><term>Animaux</term><term>Culicidae (parasitologie)</term><term>Filariose lymphatique (épidémiologie)</term><term>Humains</term><term>Lutte contre les moustiques ()</term><term>Prévalence</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (normes)</term><term>Vecteurs de maladie</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN des helminthes</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mosquito Control</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culicidae</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Vectors</term><term>Humans</term><term>Prevalence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Lutte contre les moustiques</term><term>Prévalence</term><term>Réaction de polymérisation en chaîne</term><term>Vecteurs de maladie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">PCR has recently been studied as a promising tool for monitoring the progress of efforts to eliminate lymphatic filariasis. PCR can be used to test concurrently at least 30 pools, with as many as 40 mosquitoes in each pool, for the presence of filarial larvae. The SspI PCR assay for the detection of Wuchereria bancrofti DNA in pools of mosquitoes has been used since 1994 in a variety of laboratories worldwide. During that time, the original assay has been modified in these different laboratories and no standardized assay currently exists. In an effort to standardize and improve the assay, a meeting was held on 15-16 November 2001, at Emory University in Atlanta, with representatives from most of the laboratories currently using the assay. The first round of testing was designed to test the four most promising methods for DNA extraction from pools of mosquitoes. Two of the four methods stood out as clearly the best and these will be now optimised and evaluated in two further rounds of testing.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Bierwert, L A" sort="Bierwert, L A" uniqKey="Bierwert L" first="L A" last="Bierwert">L A Bierwert</name><name sortKey="Boakye, D A" sort="Boakye, D A" uniqKey="Boakye D" first="D A" last="Boakye">D A Boakye</name><name sortKey="Fischer, P" sort="Fischer, P" uniqKey="Fischer P" first="P" last="Fischer">P. Fischer</name><name sortKey="Goodman, D" sort="Goodman, D" uniqKey="Goodman D" first="D" last="Goodman">D. Goodman</name><name sortKey="Helmy, H" sort="Helmy, H" uniqKey="Helmy H" first="H" last="Helmy">H. Helmy</name><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name><name sortKey="Lammie, P J" sort="Lammie, P J" uniqKey="Lammie P" first="P J" last="Lammie">P J Lammie</name><name sortKey="Laney, S J" sort="Laney, S J" uniqKey="Laney S" first="S J" last="Laney">S J Laney</name><name sortKey="Ottesen, E A" sort="Ottesen, E A" uniqKey="Ottesen E" first="E A" last="Ottesen">E A Ottesen</name><name sortKey="Plichart, C" sort="Plichart, C" uniqKey="Plichart C" first="C" last="Plichart">C. Plichart</name><name sortKey="Ramzy, R M R" sort="Ramzy, R M R" uniqKey="Ramzy R" first="R M R" last="Ramzy">R M R. Ramzy</name><name sortKey="Saunders, L J" sort="Saunders, L J" uniqKey="Saunders L" first="L J" last="Saunders">L J Saunders</name><name sortKey="Vasuki, V" sort="Vasuki, V" uniqKey="Vasuki V" first="V" last="Vasuki">V. Vasuki</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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